何其华：博士，北京大学医学部教授级高级工程师。北京大学医药卫生分析中心生物成像与分析实验室副主任，兼任北京电镜学会副理事长，中国电镜学会理事及激光共聚焦专业委员会副主任，生物医学工程光子学分会委员。

教育经历：北京大学，细胞生物学，博士；北京大学，生物物理学，硕士；浙江大学，生物医学工程，学士。

工作经历：长期致力于生物成像技术应用研究，包括激光共聚焦成像、双光子成像、超高分辨成像和太赫兹成像，负责激光扫描共焦显微镜Confocal平台和图像分析平台的建立、建设及管理。搭建了传统单光子共聚焦平台，转盘共聚焦显微镜平台、正置双光子和单分子FCS平台、倒置双光子和荧光寿命成像检测平台及STED超高分辨成像和SMD单分子检测平台。获得过三次北京大学实验技术成果奖，两次为二等奖，一次为三等奖。为研究生和医学实验班本科生开设相关技术课程。编写十多万字Confocal培训教材。编写科学出版社出版《Techniques in Confocal Microscopy》英文原著的导读及前言、封底和目录的翻译，参编《细胞生物学》、《受体研究技术》等4部教材，发表SCI论文四十余篇。同时从事神经肿瘤干细胞相关研究。

主持或主要参加科研项目:

1. 科技部国家重大仪器开发项目“相干太赫兹源科学仪器设备开发”（ 项目编号2011YQ130018）子任务“移动式QCL-THz全息成像仪的需求使用方案”（ 子项目编号2011YQ13001806）；资助金额100万；为子任务负责人。

2.国家自然科学基金(重点项目2016，项目批准号是31530084 ）”囊泡转运参与重要膜蛋白功能的调控机制”；资助经费335.8万元，在研，项目主要参与者，其中北京大学作为合作单位，本人项目经费分配为26.8万元。

3. 国家自然科学基金(面上基金2012)“TIRFM-FCS成像系统搭建及其在膜蛋白单分子应用的研究”，项目编号：31270412；资助金额：90万（本人经费13.5万）；项目主要参与者，其中北京大学作为合作单位，本人项目经费分配为13.5万元。

主要研究方向：

1.双光子在体成像技术方法学研究和应用研究（如微血管、血流、血脑屏障、血管通透性、脑、脊髓及各种脏器在体成像）

2.荧光寿命成像FLIM（FLIM-FRET）和荧光相关（互相关）光谱FCS/FCCS单

分子SMD检测方法学研究及应用研究（如蛋白质相互作用）。

3.STED超高分辨成像技术方法学研究及应用研究（如线粒体内膜、EGFRVIII转运途径）

4.太赫兹全息成像技术的胶质瘤病理检测研究

5.神经肿瘤干细胞及神经胶质瘤EGFR和EGFRVIII逆转运途径及成瘤机制研究等

近几年发表的主要文章：

一作和通讯作者：

1. Zhou S, Xia Y, Liu Y, He Q*, Song B*. Aggregation Induced Emission Fluorogens Light Cells via Microtubules: Accessing the Mechanisms of Intracellular Trafficking of Ionic Substances. Langmuir. 2017 Jun 13;33(23):5947-5956. doi: 10.1021/acs.langmuir.6b04301. Epub 2017 May 31.

2. Sun H, Zhang M, Cheng K, Li P, Han S, Li R, Su M, Zeng W, Liu J, Guo J, Liu Y，Zhang X, He Q*, Shen L* Resistance of glioma cells to nutrient-deprived microenvironment can be enhanced by CD133-mediated autophagy. Oncotarget. 2016 Oct 21. （IF 5.0）

3. Liu, Xu-Jie ，Wu, Wen-Tao，Wu, Wei-Hua，Yin, Feng，Ma, Si-Hai，Qin,

Jia-Zhen，Liu, Xiu-Xiu，Liu, Yi-Nan，Zhang, Xiao-Yan，Li, Peng，Han, Shuo，Liu,Kai-Yu，Zhang, Jin-Ming，He, Qi-Hua* ，Shen, Li* .A minority subpopulation of CD133(+) /EGFRvIII(+) /EGFR(-) cells acquires stemness and contributes to

gefitinib resistance.，CNS Neurosci Ther，2013.7.01，19（7）：494~502

4. Weihua Wu1,2*,Qihua He2,3*, Xiaoxia Li2,4*, Xiaoyan Zhang1,2, Aili Lu1,2, Ruimin Ge1,2, HongYing Zhen1,2, Al-fred E. Chang5, Qiao Li5, Li Shen1,2. Long-term Cultured Human Neural Stem Cells Undergo Spontaneous Transformation to Tumor-Initiating Cells. Int. J. Biol. Sci. 2011; 7(6):892-901

5. Zhen HY*, He QH*, Li Y, Zhou J, Yao C, Liu YN, Ma LJ. Lidamycin induces neural differentiation of mouse embryonic carcinoma cells through down-regulation of transcription factor Oct4. Biochem Biophys Res Commun. 2012 Apr 27;421(1):44-50. doi: 0.1016/j.bbrc.2012.03.105. Epub 2012 Mar 27.

6. Hong-Ying Zhen*, Qi-Hua He*, Yong-Zhan Zhen, Shu-Ling Wang. Yi-Nan Liu ,Wei-Hua Wu , Xiao-Yan Zhang. Ai-Li Lu , Li Shen. Inhibition of mouse embryonic carcinoma cell growth by lidamycin through down-regulation of embryonic stem cell-like genes Oct4, Sox2 and Myc. Invest New Drugs（2011）29-1188-1197 DOI 10.1007/s10637-010-9463-x. (IF 2.47)

7. Wen Jing Lu1, Feng Lan1, Qihua He1, Andrew Lee3, Chad Z. Tang, Lin Dong, Baojin Lan, Xiaowen Ma, Joseph C. Wu and Li Shen*. Inducible expression of stem cell associated

intermediate filament nestin reveals an important role in glioblastoma Carcinogenesis. Int J Cancer. 2011 Jan 15;128(2):343-51.

合作发表文章:

1. Li R, He Q, Han S, Zhang M, Liu J, Su M, Wei S, Wang X, Shen L.MBD3 inhibits formation of liver cancer stem cells.Oncotarget. 2017 Jan 24;8(4):6067-6078. doi: 10.18632/oncotarget.13496.

2. Xin Cong, Zhang Y, Qihua He,Wei T, Zhang XM,Xiang RL, Yu GY, Wu LLet al. Endothelial tight junctions are opened in cholinergic-evoked salivation in vivo. Journal of Dental Research, 2017 May;96(5):562-570（Cover Image）

3. Li X, Xing J, Qiu Z, He Q, Lin J. Quantification of Membrane Protein Dynamics and Interactions in Plant Cells by Fluorescence Correlation Spectroscopy. Molecular Plant. 2016 Sep 6;9(9):1229-39. （IF7.142）

4. Han S, Guo J, Liu Y, Zhang Z, He Q, Li P, Zhang M, Sun H, Li R, Li Y, Zeng W, Liu J, Lian L, Gao Y, Shen L. Knock out CD44 in reprogrammed liver cancer cell C3A increases CSCs stemness and promotes differentiation.. Oncotarget. 2015 Oct 22. doi: 10.18632/oncotarget.6090. Epub ahead of print

5. Lu S, Yang Y, Du Y, Cao LL, Li M, Shen C, Hou T, Zhao Y, Wang H, Deng D, Wang L, He Q, Zhu WG.The transcription factor c-Fos coordinates with histone lysine-specific demethylase 2A to activate the expression of cyclooxygenase-2. Oncotarget. 2015 Oct 27;6(33):34704-17. doi: 10.18632/oncotarget.5474.

6. Kaowen Yan , Li Li, Xiaojian Wang, Ruisha Hong, Ying Zhang, Hua Yang, Ming Lin，Sha Zhang, Qihua He, Duo Zheng, Jun Tang, Yuxin Yin, Genze Shao. .The deubiquitinating enzyme complex BRISC is required for proper mitotic spindle assembly in mammalian cells. JCB 2015( Accepted) IF(9.786)

7. Wang Z1, Hu J, Li G, Qu L, He Q, Lou Y, Song Q, Ma D, Chen Y*. PHF23 (plant homeodomain finger protein 23) negatively regulates cell autophagy by promoting ubiquitination and degradation of E3 ligase LRSAM1. Autophagy. 2014;10(12):2158-70. doi: 10.4161/auto.36439 (IF 11.423)

8. Liu Y, Li P, Liu K, He Q, Han S, Sun X, Li T, Shen L*. Timely inhibition of Notch signaling by DAPT promotes cardiac differentiation of murine pluripotent stem cells. PLoS One. 2014 Oct 14;9(10):e109588. doi: 10.1371/journal.pone.0109588. eCollection 2014. (IF 4)

9. Zhou T, Zhao L, Zhan R, He Q, Tong Y, Tian X, Wang H, Zhang T, Fu Y, Sun Y, Xu F, Guo X, Fan D, Han H, Chui D. Blood-brain barrier dysfunction in mice induced by lipopolysaccharide is attenuated by dapsone. Biochem Biophys Res Commun. 2014 Oct 24;453(3):419-24. doi: 10.1016/j.bbrc.2014.09.093. Epub 2014 Sep 28

10. Huaiqing Hao,a,1 Lusheng Fan,a,1 Tong Chen,a Ruili Li,b,c Xiaojuan Li,b,c Qihua He,d Miguel A. Botella,e and Jinxing Linb,c,2. Clathrin and Membrane Microdomains CooperativelyRegulate RbohD Dynamics and Activity in ArabidopsisC. The Plant cell

2014 ( IF 9.575)

11. Zhu, K Yan1, L Li, M Lin1, S Zhang, Q He, D Zheng, H Yang and G Shao*. K63-linked ubiquitination of FANCG is required for its association with the Rap80-BRCA1 complex to modulate homologous recombination repair of DNA interstand crosslinks. Oncogene (2014), 1–12 (IF 8.314)

12. Zhou T, He Q, Tong Y, Zhan R, Xu F, Fan D, Guo X, Han H, Qin S, Chui D*. Phospholipid transfer protein (PLTP) deficiency impaired blood-brain barrier integrity by increasing cerebrovascular oxidative stress. Biochem Biophys Res Commun. 2014 Mar 7;445(2):352-6. (IF 2.474)

13. Wang Q, Zhao Y, Luo W, Li R, He Q, Fang X, Michele RD, Ast C, von Wirén N, Lin J*. Single-particle analysis reveals shutoff control of the Arabidopsis ammonium transporter AMT1;3 by clustering and internalization. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13204-9

14. Wang H, Zhou W, Zheng Z, Zhang P, Tu B, He Q, Zhu WG. The HDAC inhibitor depsipeptide transactivates the p53/p21 pathway by inducing DNA damage DNA Repair (Amst). 2012 Feb 1;11(2):146-56. Epub 2011 Nov 23.

15. Li X, Wang X, Yang Y, Li R, He Q, Fang X, Luu DT, Maurel C, Lin J. Single-molecule analysis of PIP2;1 dynamics and partitioning reveals multiple modes of Arabidopsis plasma membrane aquaporin regulation. Plant Cell. 2011 Oct;23(10):3780-97. doi: 10.1105/tpc.111.091454. Epub 2011 Oct 18.

16. Li G, Liang JM, Li PW, Yao X, Pei PZ, Li W, He QH, Yang X, Chan QC, Cheung PY, Ma QY, Lam SK, Cheng PY, Yang ES* . Physiology and cell biology of acupuncture observed in calcium signaling activated by acoustic shear wave. Pflugers Arch. 2011 Oct;462(4):587-97. doi: 10.1007/s00424-011-0993-7. (IF 3. 7)